Antagonistic Effect of Probiotics on Drug Resistant Pseudomonas aeruginousa isolated from Burn Wound Infection in Mazandaran Province

Background and Objective: Pseudomonas aeruginosa is an opportunistic bacterium and is one of the main causes of infection in burn patients. As this bacterium gets resistant to various types of antibiotics through different acquiring mechanisms, it can easily result in skin deformities and even patients’ death. Methods: This descriptive study was carried out on 200 patients with burn injuries hospitalized in Specialized Burn Hospitals. Culture methods and diagnosis tests were used to separate and diagnose Pseudomonas aeruginosa, and disk diffusion Agar method with the Kirby-Bauer standard was applied to determine the pattern of drug-resistance. The antagonist effect of lactic bacteria isolated from 14 samples of milk and yogurt on the growth of Pseudomonas aeruginosa was also assessed using Agar well diffusion method. Findings: In this study, 30 strains were diagnosed and isolated as Pseudomonas aeruginosa, out of which 45.2% were resistant to Gentamicin, 51.6% to cefotaxime, 48.8 to Imipenem and 45.2 to Ciprofloxacin. While studying probiotics, it was found out that most isolated strains belonged to lactobacilli, with Lactobacillus casei, with the average diameter of 20.3 mm, having the most inhibitory effect. Conclusions: The results of this study showed that the spread of P.aeruginosa resistant to medicine is very high in hospitals and that the isolated Lactobacilli have a better inhibitory effect on drug resistant P.aeruginosastrains in comparison with other lactic bacteria. Therefore, using probiotic products would be more effective and could replace common antibiotics, and would help to treat patients with a great deal.